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However, the questioned toxicity of copper catalysts, even though well-established protocols of purifications (i.e., dialysis techniques) are available, has led to a strong demand of metal-free click reactions for biological applications

The carboxyl side chain of glutamic acid is able to act as a donor and acceptor of ammonia, which is toxic to the body, enabling the safe transport of ammonia to the liver where it is converted to urea and excreted by the kidneys

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Liu L, Poon R, Chen L, Frescura AM, Montuschi P, et al: Acute effects of air pollution on pulmonary function, airway inflammation, and oxidative stress in asthmatic children

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